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一步法人癌胚抗原相關(guān)細(xì)胞粘附分子1(CEACAM1)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK026ES

規(guī)格：	價(jià)格：
64T	￥2240.00
112T	￥3200.00

下載說(shuō)明 ①

Overview

Product name:	EasyStep Human CEACAM1(Carcinoembryonic Antigen Related Cell Adhesion Molecule 1) ELISA Kit
Reactivity:	Human
Alternative Names:	CD66a; BGP; BGP1; BGPI; CD66-A; Biliary Glycoprotein
Assay Type:	Sandwich
Sensitivity:	89.5 pg/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	CD & Adhesion molecule;
Uniprot ID:	P13688
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEACAM1, and the Human CEACAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEACAM1. After TMB substrate solution is added, only those wells that contain Human CEACAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEACAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.045	1.947
50.00	1.628	1.530
25.00	1.196	1.098
12.50	0.872	0.774
6.25	0.539	0.441
3.13	0.324	0.226
1.57	0.156	0.058
0.00	0.098	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CEACAM1 and the recovery rates were calculated by comparing the measured value to the expected amount of CEACAM1 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	92-105%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CEACAM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.