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人誘導型一氧化氮合成酶(NOS2/iNOS)酶聯免疫吸附檢測試劑盒

ELK2643

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Human NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
Reactivity:	Human
Alternative Names:	NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
Uniprot ID:	P35228
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

CORONARY ARTERY DISEASE

Serum malondialdehyde levels at admission as a predictor of inhospital mortality in patients with acute coronary syndrome

日期：2025-01-17 IF：1.5
CORONARY ARTERY DISEASE

Serum malondialdehyde levels at admission as a predictor of inhospital mortality in patients with acute coronary syndrome

日期：2025-01-17 IF：1.5
CORONARY ARTERY DISEASE

Serum malondialdehyde levels at admission as a predictor of inhospital mortality in patients with acute coronary syndrome

日期：2025-01-17 IF：1.5
CORONARY ARTERY DISEASE

Serum malondialdehyde levels at admission as a predictor of inhospital mortality in patients with acute coronary syndrome

日期：2025-01-17 IF：1.5

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	1.984	1.899
5.00	1.586	1.501
2.50	1.174	1.089
1.25	0.797	0.712
0.63	0.529	0.444
0.32	0.347	0.262
0.16	0.187	0.102
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	85-95%	90%
Heparin plasma(n=5)	79-93%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.