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人干擾素調(diào)節(jié)因子4(IRF4)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK9110

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human IRF4(Interferon Regulatory Factor 4) ELISA Kit
Reactivity:	Human
Alternative Names:	Interferon regulatory factor 4; IRF 4; IRF-4; Irf4; IRF4_HUMAN; LSIRF; Lymphocyte specific interferon regulatory factor; Lymphocyte specific IRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM 1; MUM1; NF EM5; NF-EM5; NFEM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP
Assay Type:	Sandwich
Sensitivity:	0.119 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Immune molecule;
Uniprot ID:	Q15306
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IRF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IRF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IRF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IRF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi

Role of interferon regulatory factors in predicting the prognosis of Crimean-Congo hemorrhagic fever

日期：2025-01-17 IF：0.6
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi

Role of interferon regulatory factors in predicting the prognosis of Crimean-Congo hemorrhagic fever

日期：2025-01-17 IF：0.6
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi

Role of interferon regulatory factors in predicting the prognosis of Crimean-Congo hemorrhagic fever

日期：2025-01-17 IF：0.6
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi

Role of interferon regulatory factors in predicting the prognosis of Crimean-Congo hemorrhagic fever

日期：2025-01-17 IF：0.6

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	1.954	1.869
10.00	1.597	1.512
5.00	1.053	0.968
2.50	0.877	0.792
1.25	0.538	0.453
0.63	0.381	0.296
0.32	0.213	0.128
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IRF4 and the recovery rates were calculated by comparing the measured value to the expected amount of IRF4 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	95-107%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRF4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.