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人脫-γ羧基凝血酶原(DCP)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK9513

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human DCP(Des-gamma carboxyprothrombin) ELISA Kit
Reactivity:	Human
Alternative Names:	CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
Assay Type:	Sandwich
Sensitivity:	0.116 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DCP in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Electroanalytical Chemistry

Rapid detection of des-gamma-carboxy prothrombin with disposable screen printed ITO electrode based on multiple signal amplification strategy of metal–organic skeleton material

日期：2025-01-17 IF：4.1
Journal of Electroanalytical Chemistry

Rapid detection of des-gamma-carboxy prothrombin with disposable screen printed ITO electrode based on multiple signal amplification strategy of metal–organic skeleton material

日期：2025-01-17 IF：4.1
Journal of Electroanalytical Chemistry

Rapid detection of des-gamma-carboxy prothrombin with disposable screen printed ITO electrode based on multiple signal amplification strategy of metal–organic skeleton material

日期：2025-01-17 IF：4.1
Journal of Electroanalytical Chemistry

Rapid detection of des-gamma-carboxy prothrombin with disposable screen printed ITO electrode based on multiple signal amplification strategy of metal–organic skeleton material

日期：2025-01-17 IF：4.1

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.231	2.146
10.00	1.612	1.527
5.00	1.128	1.043
2.50	0.933	0.848
1.25	0.594	0.509
0.63	0.387	0.302
0.32	0.258	0.173
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant DCP and the recovery rates were calculated by comparing the measured value to the expected amount of DCP in samples.

Matrix	Recovery range	Average
serum(n=5)	79-93%	86%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	79-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DCP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.